Increasing the productivity per unit area through crop management is one of the most important factors affecting yield optimization. Basically, corn breeders are interested in obtaining genotypes that are desirable in terms of functional traits and other agricultural traits. Optimal leaf removal and appropriate row spacing through improving light distribution and photosynthetic capacity associated with increases in grain yield. The amount of corn yield per hectare varies in different areas and depends on the environmental conditions, crop variety, the amount of fertilizer consumption, irrigation and fight against pests and diseases and many other conditions. Increasing productivity per unit area through agronomic management is one of the most important factors affecting yield optimization. Defoliation and appropriate row spacing are associated with increased grain yield by improving light distribution and photosynthetic capacity. Plant height, stem diameter, plant dry weight, and leaf dry weight decreased with increasing levels of defoliation and planting row spacing during reproductive stages. The traits related to corn seed yield were significantly influenced by the spacing of planting rows and defoliation levels and their interaction. In this study, field experiments were conducted in randomized complete block design based on split plots to analyze the effects of defoliation (control, no leaf removal, 100% leaf removal at the 7-leaf stage, 50% leaf removal at the 14-leaf stage, 100% leaf removal at the 14-leaf stage; 50% leaf removal at the blister stage, and 100% leaf removal at the blister stage) and row spacing (40 and 70 cm) on morphological traits of single-cross hybrid corn cultivars Karun 701 and Kosha Single-Cross 201 during 2019 and 2020 in Bam city. Plant height, stem diameter, plant dry weight, and leaf dry weight decreased with increasing defoliation levels and row spacing during the reproductive stages. The results obtained from the total of two consecutive years of the experiment indicated that the effect of planting pattern on corn grain yield was highly significant at the 5% statistical level and the effect of cultivars on corn grain yield was highly significant at the 1% statistical level. The traits related to corn grain yield were significantly affected by row spacing and defoliation levels and their interaction. The highest grain yield in the first and second years was 13505.51 and 17952.60 kg/ha, respectively, in the treatment of 50% leaf removal at the blister stage. 50% leaf removal at the blister stage and 70 cm row spacing are recommended as suitable treatments for cultivation in the region. Our results indicated that different cultivars had different responses to plant density by adjusting yield components. Leaf removal can increase both total dry-matter accumulation and thus also grain yield. The mentioned results in agreement with previous international research. Also, optimal leaf removal and row spacing improved light distribution, increased photosynthetic capacity and thus the grain yield.According to the climatic conditions of each region and the characteristics of cultivars, one of the important factors for more production per unit area is choosing the appropriate density. Overall, the 50% leaf removal in blister stage, 70 cm row spacing, and Karun and Koosha variety are recommended as the suitable treatment and variety for cultivation in the area.