مطالعه تحمل فیزیولوژیکی چغندرقند به تنش خشکی با مصرف اسید سالیسیلیک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه کشاورزی، دانشگاه پیام نور، تهران، ایران

2 دانش آموخته کارشناسی ارشد دانشگاه پیام نور، تهران، ایران

چکیده

به منظور تأثیر مصرف اسید سالیسیلیک بر افزایش تحمل فیزیولوژیکی چغندرقند به تنش خشکی، آزمایشی به صورت اسپلیت پلات فاکتوریل و در قالب طرح پایه بلوک‎های کامل تصادفی با سه تکرار در شهرستان جوین انجام شد. تیمار‎ها شامل آبیاری کامل و تنش خشکی (50 درصد آبیاری کامل)، و محلول‎پاشی اسید سالیسیلیک در سه سطح صفر، 2/5 و پنج میلی‎مولار روی رقم‎های 034(شکوفا) و 031(آریا) بود. نتایج نشان داد که مصرف اسید سالیسیلیک عملکرد ریشه و عملکرد قند ناخالص را افزایش و نیتروژن، سدیم و پتاسیم مضر (در شرایط تنش) را کاهش داد. اسید سالیسیلیک به عنوان یک ماده ضد تنش با تأثیر مثبت بر افزایش محتوای نسبی آب برگ و اسید آمینه پرولین و هم‎چنین کاهش درصد نشت الکترولیت برگ (در شرایط آبیاری کامل)، میزان عملکرد ریشه و عملکرد قند ناخالص (عیار) را در هر دو شرایط تنش خشکی و آبیاری کامل افزایش داد. عملکرد ریشه در شرایط آبیاری کامل با مصرف 2/5 و پنج میلی‎مولار اسید سالیسیلیک در رقم 034 از 20/7 (در شرایط عدم مصرف اسید سالیسیلیک) به ترتیب به 22/5 و 27/6 کیلو گرم بر متر مربع رسید. رقم 031 در شرایط تنش خشکی و مصرف پنج میلی‎مولار اسید سالیسیلیک بیشترین عملکرد ریشه را تولید کرد در صورتی‎که رقم 034 در شرایط آبیاری کامل و مصرف پنج میلی‎مولار اسید سالیسیلیک بیشترین عملکرد ریشه را تولید کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Study of physiological tolerance of sugar beet to drought stress with salicylic acid consumption

نویسندگان [English]

  • Abbas Abhari 1
  • Majid Seydabadi 2
  • Mansore Kermani 1
1 Department of Agricultural Sciences, Payame Noor University, Tehran, Iran
2 MSc Graduate, Department Agricultural Sciences, Payame Noor University, Tehran, Iran
چکیده [English]

Introduction: Environmental factors affect the accumulation of sugar. Light, sunny hours and soil moisture largely determines how sugar is grown and stored at the root of sugar results of the experiment on sugar beet showed that all treatments of anti-stress materials such as salicylic acid, thioufol and glycine at different concentrations increased the quality traits such as root yield, sugar level and white sugar yield. Reducing membrane damage due to the use of salicylic acid is associated with the production of antioxidants, and thus salicylic acid increases the membrane stability against oxidative stress. Salicylic acid is an internal regulator of plant growth that affects metabolic and physiological responses to plant growth and development.
Plants obtained from seeds pretreated with salicylic acid can improve the rate of photosynthesis in normal and stress conditions by increasing the relative water content, transpiration intensity, stomatal conductance and leaf chlorophyll index. In a study, it has been observed that salicylic acid treatment controls polysaccharide hydrolyzing enzymes and accelerates the formation of polysaccharides from soluble sugars. With this assumption, salicylic acid increases the amount of insoluble sugars compared to soluble sugars.
Materials and Methods: In order to adjust the negative effects of drought stress in sugar beet cultivars under the influence of salicylic acid foliar spraying in drought stress conditions, a factorial split plot was used in a randomized complete block design with three replications in Jouin was conducted in 2019. The studied factors included irrigation at two levels of full irrigation and drought stress (50% of full irrigation), salicylic acid spraying at three levels of 0, 2.5 and 5 mM on the Shokofa (034) and Aria (031).
Results and Discussion: Stress reduced the yield, but the use of salicylic acid increased the yield. Application of 5 mM produced the highest amount of root yield with 16.55. In the condition of full irrigation, the yield increased by increasing the amount of salicylic acid. In the stress conditions, the percentage of impure sugar increased, but in the condition of full irrigation, the percentage of impure sugar decreased, but with the use of five to 2.5 mM salicylic acid, it increased.
In condition of drought stress and consumption, 2.5 mM of salicylic acid, 034, have the highest percent sugar content with 20.5%. The amount of proline of 034 cultivar was the highest in drought stress and consumption of 5 mM of salicylic acid. Consumption of salicylic acid increased root yield and sugar content yields and reduced harmful nitrogen and sodium.
Drought stress caused a decrease in yield, and this decrease in yield was both qualitative and quantitative. Drought stress decreased sugar yield and root yield in sugar beet by increasing electrolyte leakage from cell membranes, decreasing relative leaf water content, and increasing harmful nitrogen.
Conclusion: The amount of impure sugar (kg/m2) increased with the use of salicylic acid in both drought stress and complete irrigation. Impure sugar, which is the result of multiplying the sugar beet root yield by the percentage of impure sugar and has been affected by salicylic acid, shows that the quantity and quality of both have increased with the use of this hormone-like substance. In this study, salicylic acid was able to increase the absorption of water by increasing the proline and making the osmotic pressure of sugar beet negative by increasing the relative water content of the leaves, and finally, by increasing the impute sugar yield, it increased the root yield and sugar per unit area. The consumption of salicylic acid increased the root yield and sugar level and decreased harmful nitrogen, sodium and potassium.

کلیدواژه‌ها [English]

  • Electrolyte Leakage
  • Nitrogen Content
  • Root Yield
  • Sugar Content
Arfan, M., Athar, H.R. and Ashraf, M., 2007. Does exogenous application of Salicylic Acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? Journal of Plant Physiology, 164, PP.685-694. doi: 10.1016/j.jplph.2006.05.010
Abhari, A. and Gholinezhad, A., 2019. Effect of salicylic acid foliar application on barley water use efficiency in cut-off condition. Iranian Agricultural Research Journal, 17(1), PP.157-167 [In Persian]. doi: 10.22067/gsc.v17i1.73912
Alayi Moghadam, SH., Esmaili, M.A., Rajabi, A. and Najafi, H., 2018. Effects of water deficit stress on physiological and biochemical traits of sugar beet genotypes (Beta vulgaris L.). Journal of Sugar Beet, 34(2), PP.131-146 [In Persian].  doi:10.22092/jsb.2019.109072.1139
Borsanio, V. and Botella, M.A., 2001. Evidence for a role of Salicylic Acid in the oxidative damage generated by Nacl and osmotic stress in Arabidodsis stress in (Arabidopsis) seedlings. Plant Physiology, 126, PP.1024-1030. doi: 10.1104/pp.126.3.1024
Bayat, A. 1995. Effects of different irrigation regimes on quantitative and qualitative properties of sugar beet. The Research Report of Khorasan Sugar Beet Seed Improvement Department.  PP.97-92.
Draycott, A.P. and Christenson, D.R., 2003. Nutrients for sugar beet production Soil- Plant relationships. CABI, PP.7-32.
Draycott, A.P. 2008. Sugar beet. John Wiley and Sons. 235 PP.
Ezzat Ahmadi, M., Noormohammadi, Gh., Ghodsi, M. and Kafi, M., 2010. Effect of water stress and spraying of potassium iodide on agronomic traits and grain yield of bread wheat (Tiriticum aistivum L.) genotypes. Iranian Journal of Field Crops Research, 8(2), PP.177-182 [In Persian]. doi: 10.22067/gsc.v8i2.7515
Ghanbari Birgani, D. and Sharifi, H., 2000. Investigating betanal progress AM for the control of broad leaf weeds in sugar beet. Final Research Report. Saffiabad Agricultural Research Station, Khuzestan, Iran [In Persian].
Gill, P.K., Sharma, A.D., Singh, P. and Bnullar, S.S., 2003. Changes in germination, growth and soluble sugar contents of Sorghum bicolor L. moench seeds under various a biotic stresses. Plant Growth Regulation, 40, PP.154-162. doi: 10.1023/a:1024252222376
Gooding, M., Ellis, R., Shewry, P. and Schofield, J., 2003. Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat. Journal of Cereal Sciences, 37, PP.295-309. doi:10.1006/jcrs.2002.0501
Ghoulam, C.F., Ahmed, F. and Khalid, F., 2001. Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environmental & Experiment Botany, 47, PP.139-150. doi:10.1016/S0098-8472(01)00109-5
Hayat, Q., Hayat, S., Irfan, M. and Ahmad, A., 2010. Effect of exogenous Salicylic Acid under changing environment: A review. Environmental & Experimental Botany, 68, PP.14-25. doi:10.1016/j.envexpbot.2009.08.005
Hayat, S. and Ahmad, A., 2005. Salicylic acid: A plant hormone. Springer. 97-99.
Khajepour, M.R., 2013. Industrial plants. Publications of Isfahan Industrial Unit, page 328.
Kheirkhah, M., Farazi, M., Dadkhah, A. and Khoshnood, A., 2016. Application of glycine, tufool and salicylic acid in sugar beet (Beta vulgaris L.) under drought conditions. Journal of Crop Ecophysiology, 10(1), PP.167-182 [In Persian].
Kiani, A.R., 2010. Optimal irrigation scheduling based on water-yield relations in some soybean cultivars. Journal of Agriculture Engineering Research, 11(1), PP.85-102.
Muneera, D.F.A., Yaser, M.H., Kotb, A., Emadeldeen, R., Latifa, A., Hussah, I.M.A. and Khaled, A.A.A., 2021. Evaluation of silicon and proline application on the oxidative machinery in drought-stressed sugar beet. Antioxidants, 10(398), PP.1-19. doi: 10.3390/antiox10030398
Naghizadeh, M. and Gholami Tooran Poshti, M. 2014. Evaluation the effect of seed priming by salicylic acid on yield and yield components of wheat under drought stress conditions. Journal of Agroecology, 6(1), PP.162-170 [In Persian]. doi: 10.22067/jag.v6i1.35683
Pak Mehr, A., Shakeri, F. and Rastgo, M., 2015. The effect of seed priming with Salicylic Acid on some photosynthetic traits of cowpea under water deficit in flowering stage. Journal of Iranian Pulses Research, 5(2), PP.19-30 [In Persian]. doi:10.22067/ijpr.v1393i2.46920
Ranjbar, M., Larry Yazdi, H. and Bromandjazi, Sh., 2010. The effect of Salicylic Acid on antioxidant enzymes, photosynthetic pigments of sugar content in rape (Brasica napus) under Pb stress. Journal of Plant Biology, 3(9), PP.39-52 [In Persian]. dor: 20.1001.1.20088264.1390.3.9.5.5
Raskin, I., 1992. Role of salicylic acid in plants. Annual Review of Plant Physiology & Plant Molecular Biology, 43, PP.463-439. doi:10.1146/annurev.pp.43.060192.002255
Shabiri, S., Ghasemi Golazani, K., Golchin, A. and Saba, J., 2006. Effect of irrigation water on phenology and yield of three chickpea cultivars (Cicer arietinum L.). Journal of Agricultural Knowledge, 16(2), PP.137-147 [In Persian]. doi:10.22034/saps.2021.12816
Saeed Abadi, H., Afshar Menesh, G., Shirzadi, M.H. and Sadegh Zadeh, S., 2021. The effect of drought stress and transplanting method on agronomic characteristics, quantitative and qualitative yield as well as water use efficiency of different sugar beet cultivars in autumn planting. Sugar Beet Journal, 37)1(, PP.27-48 [In Persian]. doi: 10.22092/jsb.2022.352225.1252
Sajedi, N.A. and Gholinezhad, A. 2012. Response of yield and yield component of dry land wheat cultivars to salicylic acid and selenium.  Iranian Journal of Field Crops Research, 10(3), PP.614-621 [In Persian]. doi: 10.22067/gsc.v10i3.17825
Simanne, B.P.C., Peacock, J.M. and Struk, P.C., 1993. Differences in development plasticity and growth rate among drought resistance and susceptible cultivar of durum wheat (Triticum Turgidum L. var. durum). Plant & Soil, 157, PP.155-166.
Smart, R.E. and Bingham, G.E., 1974. Rapid estimates of relative water content. Plant Physiology, 53, PP.258-260.
Valentovic, P., Luxova, M., Kolarovic, L. and Gasparikova, O., 2006. Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil Environment, 52(4), PP.186-191. doi:10.17221/3364-pse
Vahabi, N., Emam, Y. and Pirasteh-Anosheh, H., 2017. Improving wheat growth and yield using chlormequat chloride, salicylic acid and jasmonic acid under water stress. Iranian Journal of Field Crops Research, 15(1), PP.124-135 [In Persian]. doi:10.22067/gsc.v15i1.47584
Yadollahi, N., Mahmoud, F., Tadayon, R. and Karimi, M., 2021. The effect of potassium (K) and boron (B) foliar application on morphophysiological responses and root yields of Sugar Beet (Beta vulgaris L.) under drought stress conditions. Plant Process & Function, 42(10), PP.91-114 [In Persian]. doi: 10.47176/jcpp.11.1.209113
Yun-Xia, G., Li-Jun, Z., Feng-Hai, L., Zhi-Bin, C., Che, W., Yun-Cong, Y., Zhen-Hai, H., Jie, Z. and Zhen-Sheng, S., 2010. Relationship between jasmonic acid accumulation and senescence in drought-stress. African Journal of Agriculture Research, 5, PP.1978-1983. doi: 10.5897/ajar.9000585
Zargerian, M., Tehranifar, A., Nemati, H. and Siavashpour, B., 2016. The effect of Salicylic Acid on some morphophysiologic characteristics of sunflower seedlings in drought stress conditions. Journal of Horticulture, 30(1), PP.162-151 [In Persian].  doi:10.22067/jhorts4.v30i1.47987
Zbiec, J., Karezmarczyk, S. and Podsiadle, C., 2003. Response of some cultivated plants to methanol as compared to supplementaural irrigation. Electronic Journal of Polish Agricultural Universities, 6(1), PP.1-71.