عنوان مقاله [English]
This study was conducted to evaluate the effect of biological elicitors, chitosan and salicylic acid, on the quantitative and qualitative characteristics of Thymus daenensis in factorial experiment based on a completely randomized design with three replications during the 2016-2017 crop year at the institute of agricultural research in university of Zabol. Experimental factors included the first factor, chitosan, and the second factor, salicylic acid (four levels: 0, 0.5, 1 and 1.5 mM). The effect of chitosan, salicylic acid, and their interactions was significant on morphological and phytochemical parameters. The highest amounts of fresh weight, dry weight, inflorescence length, chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids traits were obtained in combined use of salicylic acid and chitosan treatment (1.5 mM). The highest total phenol content (67.91 mg GA / g DW) obtained from chitosan and salicylic acid (with concentrations of 1 and 1.5 mM, respectively). The highest amount of total flavonoids (4.79 mg Q / g DW) was obtained in the simultaneous application of chitosan and salicylic acid (1.5 and 1 mM, respectively). The highest antioxidant activity (98.13 %) measured in the treatment of chitosan and salicylic acid (both of them in 1 mM concentration). Also, the highest amount of essential oil (1.33 % V/ W) was obtained in elicitation with salicylic acid (1.5 mM) which showed a 47% increase compared to the control. The application of these elicitors can increase the quantitative and qualitative yield of Thymus daenensis and it is an effective step towards achieving sustainable agricultural goals.