نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل
2 گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل
3 بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
In order to identify genomic regions controlling the agro-morphological characteristics and markers linked to them under normal and salinity stress conditions, an experiment with 136 double haploid lines of barley and their parents (Nure and Tremois) was conducted based on alpha lattice design with two replications at the Agricultural Research Center of Zabol, during 2020-2021 crop year. Agronomic traits including tiller number per plant, spike number per plant, grain number per spike, awn length, internode length, node number, plant height, 1000-grain weight and grain yield were measured. There were significant differences among the genotypes for all studied traits and maximum correlation were observed between grain yield with tiller and spike number per plant. QTL analysis was conducted by composite interval mapping (CIM) method for each of the normal and stress conditions and their averages separately. A total of 24 QTLs for studied traits were detected, which explained 8 to 16% of the phenotypic variance (R2). The highest LOD scores were obtained for the trait of number of grains in spike on chromosome 2H in stress condition. Of the 7 major QTLs identified in this study, the most obvious QTL was for the number of spikes/plant (Qng2Hma) on chromosome 2H linked with marker E42M38_235-2H, which explained 16% of the phenotypic variance. Only a QTL (Qtgw1H) for 1000-grain weight, linked with marker WMC1E8 was identified as stable QTL. These identified genomic regions can be used in marker assisted breeding after validation in various genetic backgrounds and environments for salinity tolerance in Barley.
کلیدواژه‌ها [English]
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