نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد بیوتکنولوژی، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه زابل
2 گروه اصلاح نباتات و بیوتکنولوژی دانشکده کشاورزی دانشگاه زابل
3 استادیار، گروه آبیاری و زهکشی، دانشگاه زابل
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Water stress alters plant behavior through its effect on plant metabolism, therefore has a great impact on plant production. In order to evaluate the effect of drought stress on physiological and biochemical traits of milk thistle, treatments of 100%, 75%, 50% and 25% water requirement were applied in the greenhouse of Shandel village, Hirmand city. Harvest was done at different stages including 6 and 13 weeks after planting. Traits such as dry weight, fresh weight, chlorophyll a, chlorophyll b, carotenoids, proteins and proline levels and the activity of antioxidant enzymes were measured. The results of analysis of variance showed significant differences in harvest time, different irrigation levels and the interaction of harvest time and different irrigation levels. Carotenoid levels began to decrease with increasing stress intensity in both harvest stages. The amount of chlorophyll a in the stage 6 weeks after planting was less than 13 weeks after planting, but in both stages, its amount decreased with increasing stress. Protein and proline levels also increased with increasing stress intensity. The activity of antioxidant enzymes also increased with increasing stress intensity, so that in 100% of water requirement, reached the minimum value and in 25% of water requirement reached the maximum value. The results of this study showed milk thistle has a good adaptation to drought stress, which is probably due to the reduction of oxidative damage by induction of antioxidant system and accumulation of osmolytes such as proline and protein, which changes are a kind of adaptation to stress conditions.
کلیدواژه‌ها [English]
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