In order to evaluate the allelic diversity of Glutenin Subunits in estimating the quality of bread wheat, 29 promising dryland wheat genotypes were studied by SDS-PAGE. It was found that for Glu-A1 locus, 1 allele was observed for Karim, Rasad, Sabalan, Ohadi, 3, 4, 5, 8, 9, 10, 11, 14, 17, 19, 21, 22, 23 and 24  genotypes, for 6, 7, 12, 13, 16, 20, Homa and Rijaw genotypes, allele 2* and for 16, 18 and Azar2 genotypes, null allele was observed. In GLU-B1 locus, 7+9 allele was seen for Ohadi, Rijaw, 5, 9, 13, 16 and 21 genotypes, 7+8 allele was observed for Azar2, Homa, Karim, Rasad, 7, 8, 10,12, 19, 20, 21 genotypes, 17+18 allele was seen for Sabalan, 3, 4, 6, 11, 18, and 24 genotypes, and 13+16 allele was observed for 14, 15, 17 and 23 genotypes. In GLU-D1 locus, 5+10 allele was observed for Ohadi, Rijaw, Karim, 5, 9, 11, 13, 14, 15, 22 and 23 genotypes,  2+12 allele was observed for Azar2, Sabalan, Rasad, 3, 4, 6, 7, 8, 10, 12, 16, 17, 18, 19, 20, 21 and 24 genotypes. Significant Positive correlation was found between grains protein with zeleny number and significant negative correlation was observed between protein and starch percentage.  Based on principal component analysis, two factors were identified that can explain 72/8 percent of diversity.  Based on stepwise regression analysis, significant correlations were also found between null allel and hardness, and between 7+9 allel and zeleny.