ارزیابی خواص آنتی اکسیدانی و ترکیبات فنلی ژنوتیپ های مختلف انبه (Mangifera indica L) جنوب ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه پژوهشی زراعت و اصلاح نباتات، پژوهشکده کشاورزی، پژوهشگاه دانشگاه زابل، زابل، ایران

2 گروه اصلاح و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

چکیده

انبه از مهم ترین میوه های گرمسیری دنیاست که دارای ترکیبات فنلی و فلاونوئیدهای متعدد و خاصیت آنتی‌اکسیدانی می‌باشند. در این تحقیق 29 ژنوتیپ از انبه‌های بومی مناطق مختلف جنوب ایران جمع‌آوری و بر اساس میزان خواص آنتی‌اکسیدانی، ترکیبات فنلی و فلاونوئیدی در آزمایشگاه پژوهشکده زیست‌فناوری کشاورزی دانشگاه زابل در سال 1396 مورد ارزیابی قرار گرفتند. نتایج تجزیه واریانس نشان داد که ژنوتیپ‌های مختلف انبه و محیط جمع آوری انبه بر اساس میزان فنل، فلاونوئید و خواص آنتی‌اکسیدانی مختلف بودند. بیشترین میزان فلاونوئید (176/65 میلی گرم در گرم عصاره هیدرو الکلی و 122/15 میلی گرم در گرم عصاره هیدرو الکلی) به ترتیب متعلق به ژنوتیپ های هلو و خنیزی بود. بیشترین میزان فنل (11/232 و 10/664 میلی-گرم در گرم عصاره هیدرو الکلی) به ترتیب متعلق به ژنوتیپ های زاپاک و خنیزی بود. بیشترین میزان خواص آنتی‌اکسیدانی (55/240 و 50/873 میکروگرم در میلی‌لیتر) به ترتیب متعلق به ژنوتیپ های هلو و خاروست بود. بر اساس منطقه محل جمع-آوری انبه ها، بیشترین میزان فلاونوئید (76/679 و 65/126 میلی گرم در گرم عصاره هیدرو الکلی) به ترتیب متعلق به ژنوتیپ های انبه منطقه جیرفت و رودان بود. بیشترین میزان فنل (6/4783 و 6/4534 میلی گرم در گرم وزن تر برگ) به ترتیب متعلق به ژنوتیپ های انبه منطقه رودان و منوجان بود. بیشترین میزان خواص آنتی‌اکسیدانی (43/144 و 42/673 میکروگرم در میلی لیتر) به ترتیب متعلق به ژنوتیپ های انبه منطقه رودان و جیرفت بود. در بین غلظت‌های مختلف عصاره، مؤثرترین غلظت، 64 میکروگرم در میلی لیتر بود. در کل نتایج این تحقیق نشان داد که انبه‌های منطقه رودان و سپس جیرفت دارای بیشترین میزان خواص آنتی اکسیدانی بوده و با افزایش غلظت عصاره هیدروالکلی خاصیت آنتی اکسیدانی آن ها نیز بیشتر شده است.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Antioxidant Properties and Phenolic Compounds of Different Mango (Mangifera indica L.) Southern Iran

نویسندگان [English]

  • Bahman Fazeli nasab 1
  • Leila Fahmide 2
1 Research Dep. of Agronomy and Plant Breeding, Center of Agricultural Research, University of Zabol, Zabol, Iran
2 Dep. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
چکیده [English]

Mango is one of the most important tropical fruits in the world. It has many phenolic compounds, flavonoids, and antioxidants. Therefore, 29 genotypes of native mangoes in different regions of in southern of Iran (Kerman and Hormozgan) were collected and evaluated based on the amount of antioxidant, phenolic and flavonoid compounds in the laboratory of Agricultural Biotechnology Research Institute, University of Zabol in 2017. The results of analysis of variance showed that different mango genotypes and mango collection regions were affected on phenol, flavonoids and antioxidant properties. The most amount of flavonoids (176.65 mg/g of hydro alcoholic extract and 122.15 mg/g of hydro alcoholic extract) belonged to helov and Kn genotypes, respectively. The most phenol (11.232 and 10.664 mg/g of hydro alcoholic extract) belonged to Zapak and Kn genotypes. The most antioxidant properties (55.240 and 50.873 micrograms per milliliter) belonged to helov and kharvst genotypes, respectively. Based on the area where the mangoes were collected, the most amount of flavonoids (76.679 and 65.126 mg/g of hydro alcoholic extract) belonged to the mango genotypes of Jiroft and Rodan. The most phenol (6.4783 and 6.4534 mg/kg of fresh weight) belonged to Rodan and Manojan mango genotypes, respectively. The highest antioxidant properties (43.144 and 42.667 μg/ml) belonged to Rodan and Jiroft mango genotypes, respectively. Among the different concentrations of the extract, the most effective concentration was 64 μg/ml. Overall, the results showed that Mangoes in Rodan and Jiroft area had the highest antioxidant properties and increased their antioxidant properties by increasing the concentration of hydro alcoholic extract.

کلیدواژه‌ها [English]

  • Dpph
  • Phenol
  • Flavonoid
  • Hydro-alcohol
  • Plant Extract
Abbasi, M. and Heidari, M. 2010. Effect of fruit maturity on seed germination and seedling growth of mango. Journal of Crops Improvement, 12(1): 69-79.
Amzad Hossain, M. and Shah, M.D. 2015. A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian Journal of Chemistry, 8(1): 66-71. doi: 10.1016/j.arabjc.2011.01.007
Anonymous. 2015a. Statistics of the Hormozgan Agricultural Jihad Organization. Hormozgan Agricultural Organization.
Anonymous. 2015b. Surface cultivation statistics and production and yield of mango products in Iran. Ministry of Agriculture.
Baba, S.A. and Malik, S.A. 2015. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science, 9(4): 449-454. doi: 10.1016/j.jtusci.2014.11.001
Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3).
Chinnusamy, V., Zhu, J. and Zhu, J.K. 2006. Salt stress signaling and mechanisms of plant salt tolerance. In Genetic engineering. Springer. pp. 141-177.
del Bano, M.J., Lorente, J., Castillo, J., Benavente-Garcia, O., del Río, J.A., Ortuno, A., Quirin, K.W. and Gerard, D. 2003. Phenolic diterpenes, flavones, and rosmarinic acid distribution during the development of leaves, flowers, stems, and roots of Rosmarinus officinalis. Antioxidant activity. Journal of Agricultural and Food Chemistry, 51(15): 4247-4253.
Ebrahimzadeh, M.A., Hosseinimehr, S.J., Hamidinia, A. and Jafari, M. 2008. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology Online, 17-14.
Elmastaş, M., Dermirtas, I., Isildak, O. and AboulEnein, H.Y. 2006. Antioxidant Activity of S-Carvone Isolated from Spearmint (Mentha Spicata L. Fam Lamiaceae). Journal of Liquid Chromatography & Related Technologies, 29(10): 1465-1475.
Emmami, S. 2001. Culture and growing of Mango, Vol 1. The Production Office of Promotion Programs and Technical Press. 353
Fatahi moghadam, J., Hamid oghli, Y., Fotohi ghazvini, R., Ghasem Nezhad, M. and Bakhshi, D. 2011. Assessment of physicochemical and anti-oxidant of crest in some joinary cultivars of citrus. Journal of Horticultural Science, 25(2): 211-217 (In Persian).
Fazli, R., Nazarnezhad, N. and Ebrahimzadeh, M.A. 2013. Evaluation of phenols and flavonoids and antioxidant activity of the bark of beech, hornbeam, and pine. Journal of the Forest and Wood Products, 66(3): 339-342.
Fu, G., Li, W., Huang, X., Zhang, R., Tian, K., Hou, S. and Li, Y. 2018. Antioxidant and alpha-glucosidase inhibitory activities of isoflavonoids from the rhizomes of Ficus tikoua Bur. Nat Prod Res, 32(4): 399-405.
Ghasemi, K., Ghasemi, Y. and Ebrahimzadeh, M.A. 2009. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pakistan Journal of Pharmaceutical Science, 22(3): 277-281.
Gorinstein, S., Martı́n-Belloso, O., Park, Y.-S., Haruenkit, R., Lojek, A., Ĉı́ž, M., Caspi, A., Libman, I. and Trakhtenberg, S. 2001. Comparison of some biochemical characteristics of different citrus fruits. Food Chemistry, 74(3): 309-315.
He, Y. and Zhu, Z. 2008. Exogenous salicylic acid alleviates NaCl toxicity and increases antioxidative enzyme activity in Lycopersicon esculentum. Biologia Plantarum, 52(4): 792.
Hemmati, N., Ghasem Nezhad, A., Fatahi Moghadam, J. and Ebrahimi, P. 2015. The basic role in antioxidant activity citrus fruit: A Case Study compared the antioxidant activity of two cultivars of fruit juice with fruit stool. Journal of Horticultural Science, 29(2): 277-286.
Hossain, H., Shahid-Ud-Daula, A., Jahan, I.A., Nimmi, I., Hasan, K. and Haq, M.M. 2017. Evaluation of antinociceptive and antioxidant potential from the leaves of Spilanthes paniculata growing in Bangladesh. International Journal of Pharmaceutical and Phytopharmacological Research, 1(4): 178-186.
Jamshidi, M., Ahmadi-Ashteiani, H.R., Shamsali, R., Fathi Azad, F., Mazandarani, M. and Khaki, A. 2010. Analysis and Comparison of phenolic compounds and antioxidant activity of some plant species native to the Caspian. Journal of Medicinal Plants, 9(2): 177-183 (In Persian).
Jang, H.-D., Chang, K.-S., Chang, T.-C. and Hsu, C.-L. 2010. Antioxidant potentials of buntan pumelo (Citrus grandis Osbeck) and its ethanolic and acetified fermentation products. Food chemistry, 118(3): 554-558.
Kanatt, S.R., Chander, R. and Sharma, A. 2007. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chemistry, 100(2): 451-458.
Khalighi - Sigaroodi, F., Ahvazi, M., Ebrahimzadeh, H. and Rahimifard, N. 2013. Chemical Composition of the Essential Oil and Antioxidant Activities, Total Phenol and Flavonoid Content of the Extract of Nepeta pogonosperma. Journal of Medicinal Plants, 4(48): 185-198.
Khazaee, F.A., Etebarian, H.R., Roustaee, A. and Alizadeh, A. 2011. Study of Changes in Peroxidase Enzyme and Total Phenol in Golden Delicious Apple Fruits Inoculated with an Antagonistic Isolate of Pseudomonas fluorescens and Penicillium expansum the Causal Agent of Apple Blue Mould. Seed and Plant Production Journal, 26(4): 419-433. doi: 10.22092/sppj.2017.110417
Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., Hilpert, K.F., Griel, A.E. and Etherton, T.D. 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. The American Journal of Medicine, 113(9): 71-88.
Lal, S., Singh, A.K., Singh, S.K., Srivastav, M., Singh, B.P., Sharma, N. and Singh, N.K. 2017. Association analysis for pomological traits in mango (Mangifera indica L.) by genic-SSR markers. Trees, 31(5): 1391-1409.
Lee, S.E., Hwang, H.J., Ha, J.-S., Jeong, H.-S. and Kim, J.H. 2003. Screening of medicinal plant extracts for antioxidant activity. Life Sciences, 73(2): 167-179.
Maldonado, R.R., da Costa Araújo, L., da Silva Dariva, L.C., Rebac, K.N., de Souza Pinto, I.A., Prado, J.P.R., Saeki, J.K., Silva, T.S., Takematsu, E.K. and Tiene, N.V. 2017. Potential application of four types of tropical fruits in lactic fermentation. LWT-Food Science and Technology, 86254-260.
Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91(3): 571-577.
Medini, F., Fellah, H., Ksouri, R. and Abdelly, C. 2014. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science, 8(3): 216-224. doi: 10.1016/j.jtusci.2014.01.003
Mirzaei, A., Mohammadi, J., Mirzaei, N. and Mirzaei, M. 2011. The Antioxidant Capacities and Total Phenolic Contents of Some Medicinal Plants in Iran. Journal of Fasa University of Medical Sciences, 1(3): 160-167.
Mozdastan, S., Ebrahimzadeh, M.A. and Khalili, M. 2015. Comparing the Impact of Different Extraction Methods on Antioxidant Activities of Myrtle (Myrtus communis L.). Journal of Mazandaran University of Medical Sciences, 25(127): 10-24 [In Farsi].
Rakić, S., Petrović, S., Kukić, J., Jadranin, M., Tešević, V., Povrenović, D. and Šiler-Marinković, S. 2007. Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. Food Chemistry, 104(2): 830-834.
Rashedi, H., Amiri, H. and Gharezi, A. 2015. Assessment of phytochemical and antioxidant properties of the Capparis spinosa L. Khuzestan province Journal of Qazvin University of Medical Science, 18(6): 11-17.
Rehman, Z.-U. (2003). Evaluation of antioxidant activity of methanolic extract from peanut hulls in fried potato chips. Plant Foods for Human Nutrition, 58(1): 75-83.
Salhe Abadi, S. and Mehraban Sang Atash, M. 2015. Evaluation of the antioxidant activity and total phenols, flavonoids in methanolic, dichloromethane and ethyl acetate extracts of aerial parts of Rubia florida. Journal of North Khorasan University of Medical Sciences, 7(1): 101-112. doi: 10.29252/jnkums.7.1.101
Shariatifar, N., Chamanzari, H. and Ghanay M, S. 2006. The study of flos plant on progmastigote in culture. Quarterly of Horizon of Medical Sciences, 11(4): 5-9.
Sharma, R., Samant, S., Sharma, P. and Devi, S. 2012. Evaluation of antioxidant activities of Withania somnifera leaves growing in natural habitats of North-west Himalaya, India. Journal of Medicinal Plants Research, 6(5): 657-661.
Singh, S. and Singh, R. 2008. In vitro methods of assay of antioxidants: an overview. Food Reviews International, 24(4): 392-415.
Statistix, R. 2013. Statistix 10 Analytical Software. Tallahassee, FL USA.
Yeganeh Teimori, Y., Mianabadi, M.I. and Bagherieh Najjar, M.B. 2012. Antioxidant Activity of Methanolic Extract of Leaves, Stems and Roots of Satureja hortensis and Foeniculum vulgare Mill Plants. In National Symposium on Natural Products and Medicinal Plants (Bojnourd, North Khorasan University of Medical Sciences)